TYR antibody (AA 1-433)

Catalog No. ABIN573956

The Mouse Monoclonal anti-TYR antibody has been validated for WB, IF, IHC, ELISA, IHC (p) and ICC. It is suitable to detect TYR in samples from Human, Dog and Cat.

Quick Overview for TYR antibody (AA 1-433) (ABIN573956)

Target: TYR (Tyrosinase (TYR))

Reactivity: Human, Dog, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TYR antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Clone: T311

Product Details anti-TYR Antibody

Binding Specificity: AA 1-433

Brand: IHC-plus™

Specificity: Recognizes a cluster of proteins between 70-80kD, identified as human tyrosinase. Occasionally a minor band at 55kD is also detected. Does not cross-react with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Species cross-reactivity: canine and feline. Weak cross-reactivity with mouse.

Purification: Protein A purified

Immunogen: Recombinant tyrosinase protein. Epitope: 1-433.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, ICC, IF, IHC, IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for TYR

Target: TYR (Tyrosinase (TYR))

Alternative Name: Tyrosinase

Background: Name/Gene ID: TYR

Synonyms: TYR, CMM8, LB24-AB, SHEP3, SK29-AB, Monophenol monooxygenase, Tumor rejection antigen AB, OCA1A, Tyrosinase, OCAIA

Gene ID: 7299

UniProt: P14679