GAPDH antibody (AA 9-323)

Catalog No. ABIN574001

The Rabbit Polyclonal anti-GAPDH antibody is suitable to detect GAPDH in samples from Human, Mouse, Rat and Drosophila melanogaster. It has been validated for WB, IHC, IF, ICC and IHC (p).

Quick Overview for GAPDH antibody (AA 9-323) (ABIN574001)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GAPDH Antibody

Binding Specificity: AA 9-323

Specificity: Human GAPDH

Purification: Immunoaffinity purified

Immunogen: Recombinant protein fragment containing a sequence corresponding to a region within amino acids 9 and 323 of GAPDH.

Type of Immunogen: Recombinant protein

Application Details

Application Notes: Approved: ICC (1:100 - 1:200), IF (1:100 - 1:200), IHC, IHC-P (10 μg/mL), WB (1:5000 - 1:20000)

Usage: Cross-reactiviy with mouse and zebrafish has been confirmed in WB only. The antibody's suitability for use in IHC with mouse and zebrafish tissues has not been determined at this time. Cross-reactivity with rat, rabbit, cow, dog, and cat has not been experimentally confirmed at this time. The antibody is predicted to cross react with these species based on the following homology: Rat (94 % ), Rabbit (94 %), cow (94 %), Dog (95 %), Cat (96 %).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 20 % glycerol (  pH 7), 0.025 % proclin

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Name/Gene ID: GAPDH

Synonyms: GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit

Gene ID: 2597

UniProt: P04406