Reticulon 4 antibody (AA 501-515)

Catalog No. ABIN574094

The Goat Polyclonal anti-Reticulon 4 antibody has been validated for IHC and IHC (p). It is suitable to detect Reticulon 4 in samples from Human and Monkey.

Quick Overview for Reticulon 4 antibody (AA 501-515) (ABIN574094)

Target: Reticulon 4 (RTN4)

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Reticulon 4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Reticulon 4 Antibody

Binding Specificity: AA 501-515

Brand: IHC-plus™

Specificity: Human RTN4 / Nogo. This antibody is expected to recognize reported isoform A (NP_065393.1) and isoform E (NP_997404.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%) Gibbon, Panda, Horse (93%) Marmoset, Dog, Bovine, Rabbit (87%) Mouse, Rat, Bat, Pig (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KAQIVTEKNTSTKTS from an internal region of human RTN4 / Nogo (NP_065393.1, NP_997404.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%), Gibbon, Panda, Horse (93%), Marmoset, Dog, Bovine, Rabbit (87%), Mouse, Rat, Bat, Pig (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 to 5 μg/mL

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Reticulon 4

Target: Reticulon 4 (RTN4)

Alternative Name: RTN4 / Nogo

Background: Name/Gene ID: RTN4
Family: Reticulon

Synonyms: RTN4, Human NogoA, My043 protein, Neurite outgrowth inhibitor, NOGOC, NSP-CL, Nogo protein, KIAA0886, Reticulon 5, Reticulon-4, Reticulon-5, Reticulon 4, RTN4-B1, RTN-X, RTN4-A, RTN4-B2, RTN4-C, Nbla00271, Neurite growth inhibitor 220, NI220/250, NOGO-A, Nogo-C, ASY, Foocen, Nbla10545, NOGO, Nogo-B, NSP

Gene ID: 57142

NCBI Accession: NP_065393, NP_997404

UniProt: Q9NQC3

Pathways: Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome