NR1D1 antibody (AA 1-615)

Catalog No. ABIN574412

The Mouse Monoclonal anti-NR1D1 antibody has been validated for ELISA, WB, IHC, IF, IHC (p) and RNAi. It is suitable to detect NR1D1 in samples from Human and Mouse.

Quick Overview for NR1D1 antibody (AA 1-615) (ABIN574412)

Target: NR1D1 (Nuclear Receptor Subfamily 1, Group D, Member 1 (NR1D1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NR1D1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), RNA Interference (RNAi)

Clone: 4F6

Product Details anti-NR1D1 Antibody

Binding Specificity: AA 1-615

Brand: IHC-plus™

Purification: Purified from ascites by Protein A

Immunogen: NR1D1 (AAH56148, 1 a.a. ~ 615 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, IF (10 μg/mL), IHC, IHC-P (5 μg/mL), RNAi, WB

Usage: Western Blot (Cell lysate) - positive control Hela NE. Western Blot (Cell lysate) - positive control NIH/3T3. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 μg/mL) - positive control HeLa cells. Sandwich ELISA (Recombinant protein). RNAi Knockdown (Antibody validated).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4. Sourced in Ascites.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.

Target Details for NR1D1

Target: NR1D1 (Nuclear Receptor Subfamily 1, Group D, Member 1 (NR1D1))

Alternative Name: NR1D1

Background: Name/Gene ID: NR1D1
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: NR1D1, EAR1, Rev-erbA-alpha, Reverba, Reverbaalpha, Rev-erb alpha, Rev-erba, Rev-erbalpha, RevErbAlpha, THRA1, Rev-ErbAalpha, Ear-1, HRev, THRAL, V-erbA-related protein 1

Gene ID: 9572

UniProt: P20393

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha