NR1D1 antibody (AA 290-339)

Catalog No. ABIN6749732

The Rabbit Polyclonal anti-NR1D1 antibody (ABIN6749732) specifically detects NR1D1 in WB, IHC and IHC (p). The antibody is reactive with Human, Cow, Dog and Sheep samples.

Quick Overview for NR1D1 antibody (AA 290-339) (ABIN6749732)

Target: NR1D1 (Nuclear Receptor Subfamily 1, Group D, Member 1 (NR1D1))

Reactivity: Human, Cow, Dog, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR1D1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NR1D1 Antibody

Binding Specificity: AA 290-339

Specificity: Human NR1D1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog (100%) Mouse, Rat, Rabbit, Pig (92%) Sheep, Bovine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa290-339 of human NR1D1 (P20393, NP_068370). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Elephant, Panda, Dog (100%), Marmoset, Mouse, Rat, Rabbit, Pig (92%), Sheep, Bovine (85%), Horse (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 - 10 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NR1D1

Target: NR1D1 (Nuclear Receptor Subfamily 1, Group D, Member 1 (NR1D1))

Alternative Name: NR1D1

Background: Name/Gene ID: NR1D1
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: NR1D1, EAR1, Rev-erbA-alpha, Reverba, Reverbaalpha, Rev-erb alpha, Rev-erba, Rev-erbalpha, RevErbAlpha, THRA1, Rev-ErbAalpha, Ear-1, HRev, THRAL, V-erbA-related protein 1

Gene ID: 9572

NCBI Accession: NP_068370

UniProt: P20393

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha