LSD1 antibody
Quick Overview for LSD1 antibody (ABIN577699)
Target
See all LSD1 (KDM1A) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Brand
- AbX™
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Characteristics
- Reacts with mammalian Lysine Specific Demethylase 1
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Purification
- Purified
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Immunogen
- Full length recombinant Lysine Specific Demethylase 1
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Application Notes
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Western blotting and Immunoprecipitation
Western blotting, 1:50-1:200 AbX™ Anti-LSD1 Rabbit pAb
Detection of LSD1 in nuclear extract of 20 μg of HeLa cells probed with A003-100UL at a 1:200 dilution. MW of LSD1 is 110 kDa. -
Comment
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Reacts with mammalian Lysine Specific Demethylase 1 Supplied as a PBS solution Applications include Western blotting and Immunoprecipitation
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Phosphate Buffered Saline at pH 7.2 containing 0.1 % Tween 20 and 0.09 % Kathon preservative
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Preservative
- Sodium azide
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Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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Storage
- 4 °C
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Storage Comment
- Short Term: 4°C. Extended: Aliquot and freeze at -20°C
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- LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))
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Alternative Name
- Lysine Specific Demethylase 1 (LSD1)
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Background
- There are two known classes of histone demethylases (HDM), differing in their reaction chemistry, coenzyme use and reaction products. Flavin-dependent HDMs act only on mono- and dimethylated lysines and produce hydrogen peroxide, whereas the Jumonji-containing HDMs are iron-dependent enzymes that can act on mono-, di- and trimethylated lysine side chains, and also on methylated Arginine residues. Both classes also produce formaldehyde as a product of the demethylation reaction. LSD1 (Lysine-specific demethylase 1) is a flavin- dependent amine oxidase and was identified as a subunit of different complexes and has been shown to be involved in transcriptional repression of genes through the demethylation of monomethyl Lys4 and dimethyl Lys4 Histone H3. LSD1 is also involved in transcriptional activation through the demethylation of monomethyl Lys9 and dimethyl Lys9 Histone H3. It is also able to demethylate both mono- and dimethyl Lys370 in the regulatory domain of the tumor suppressor p53.
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Pathways
- Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling, Warburg Effect
Target
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