EGFR antibody (Tyr1110)

Catalog No. ABIN5956470

The Rabbit Polyclonal anti-EGFR antibody is suitable to detect EGFR in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for EGFR antibody (Tyr1110) (ABIN5956470)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-EGFR Antibody

Binding Specificity: Tyr1110

Specificity: Avian erythroblastic leukemia viral (v erb b) oncogene homolog,Cell growth inhibiting protein 40,Cell proliferation inducing protein 61,EGF R,EGFR,EGFR,Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog),Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian),Epidermal growth factor receptor,erb-b2 receptor tyrosine kinase 1,ERBB,ERBB1,Errp,HER1,mENA,NISBD2,Oncogen ERBB,PIG61,Proto-oncogene c-ErbB-1,Receptor tyrosine protein kinase ErbB 1,Receptor tyrosine-protein kinase ErbB-1,SA7,Species antigen 7,Urogastrone,v-erb-b Avian erythroblastic leukemia viral oncogen homolog,wa2,Wa5

Purification: Affinity purification

Immunogen: Synthesized peptide derived from human EGFR around the non-phosphorylation site of Tyr1110.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, ELISA 1:20000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol,  pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR

Background: Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.Isoform 2 may act as an antagonist of EGF action.

Molecular Weight: 134kDa

Gene ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins