DFFA antibody

Catalog No. ABIN5963274

The Rabbit Polyclonal anti-DFFA antibody has been validated for WB and IF. It is suitable to detect DFFA in samples from Human, Mouse and Rat.

Quick Overview for DFFA antibody (ABIN5963274)

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DFFA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-DFFA Antibody

Purification: Affinity purification

Immunogen: Recombinant protein of human DFFA

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000 IF 1:50 - 1:100

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for DFFA

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Alternative Name: DFFA

Background:

Synonyms: A330085O09Rik,Caspase activated deoxyribonuclease inhibitor short form,DFF 1,DFF 45,DFF alpha,DFF-45,DFF1,DFF35,DFF45,DFFA,Dffa DNA fragmentation factor,alpha subunit,DFFA,DNA fragmentation factor 45 kDa subunit,DNA Fragmentation Factor Alpha Subunit,DNA fragmentation factor subunit alpha,DNA fragmentation factor,45 kD,alpha subunit,DNA fragmentation factor,45  kDa,alpha polypeptide (DFFA),transcript variant 1,DNA fragmentation factor,45  kDa,alpha polypeptide,DNA fragmentation factor,alpha subunit,DNAation factor 45 kDa subunit,H13,ICAD,ICAD L,ICAD S,Inhibitor of CAD,Inhibitor of Caspase Activated DNase,MGC143066,OTTHUMP00000001903,OTTHUMP00000001904,RP23 121D17.3

Background: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Molecular Weight:

Observed_MW: 35kDa, 45kDa

Calculated_MW: 29kDa/36kDa

Gene ID: 1676

UniProt: O00273

Pathways: Apoptosis, Caspase Cascade in Apoptosis