PDPK1 antibody

Catalog No. ABIN597040

This Rabbit Monoclonal antibody specifically detects PDPK1 in WB and IP. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for PDPK1 antibody (ABIN597040)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-PDPK1 Antibody

Specificity: Recognizes human PDHK1. Species cross-reactivity: mouse, rat, monkey.

Immunogen: Synthetic peptide corresponding to the sequence of human PDHK1protein (KLH- coupled).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IP (1:50), WB (1:1000)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5 % w/v BSA, 1X TBS, 0.1 %. Tween-20 at 4 °C with gentle shaking, overnight. Immunoprecipitation: 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, < 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least1 year at -20°C.

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDK1

Background: Name/Gene ID: PDK1
Subfamily: PDK/BCKDK
Family: Protein Kinase

Synonyms: PDK1, PDHK, PDH kinase 1, PDHK1

Gene ID: 5163

UniProt: Q15118

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals