Sonic Hedgehog antibody

Catalog No. ABIN597698

The Rat Monoclonal anti-Sonic Hedgehog antibody has been validated for WB and ELISA. It is suitable to detect Sonic Hedgehog in samples from Mouse.

Quick Overview for Sonic Hedgehog antibody (ABIN597698)

Target: Sonic Hedgehog (SHH)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Sonic Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Sonic Hedgehog Antibody

Specificity: Recognizes the N-terminal peptide of human (aa24-197) and mouse (aa25-198) Shh. Cross-reactivity: N-terminal peptides of recombinant mouse Dhh (aa23-198) and rmIhh (aa66-240). No cross-reactivity with the C-terminal peptides from rmShh (aa199-437), rmDhh (aa199-396), or rmIhh (aa241-449).

Purification: Immunoaffinity purified

Immunogen: E. coli-derived recombinant mouse Sonic Hedgehog Cys25-Gly198 (Lys122Arg) (Accession # Q62226).

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, WB (1 μg/mL)

Usage: Suitable for use in ELISA and Suitable for use in Western blot: 1 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, trehalose

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Alternative Name: Sonic Hedgehog / SHH

Background: Name/Gene ID: SHH
Subfamily: Cysteine C46
Family: Protease

Synonyms: SHH, HHG-1, HLP3, Sonic hedgehog, Sonic hedgehog homolog, MCOPCB5, Sonic hedgehog protein, HHG1, HPE3, SMMCI, TPT, TPTPS

Gene ID: 6469

UniProt: Q15465

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development