Topoisomerase II alpha antibody (AA 1466-1480)

Catalog No. ABIN598126

This Rabbit Polyclonal antibody specifically detects Topoisomerase II alpha in WB. It exhibits reactivity toward Human, Pig, Dog, Horse, Rabbit and Monkey.

Quick Overview for Topoisomerase II alpha antibody (AA 1466-1480) (ABIN598126)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human, Pig, Dog, Horse, Rabbit, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: AA 1466-1480

Specificity: Recognizes human DNA topoisomerase 2-alpha at 174kD. Species cross-reactivity: rat.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Elephant, Panda, Dog, Horse, Rabbit, Pig (100%) Gibbon (93%) Bat (87%) Hamster, Opossum (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to aa1466-1480, RKPSTSDDSDSNFEK to human DNA topoisomerase 2-alpha. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Elephant, Panda, Dog, Horse, Rabbit, Pig (100%), Gibbon (93%), Bat (87%), Hamster, Opossum (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (1 - 2 μg/mL)

Usage: Suitable for use in Western blot: 1-2 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile distilled water

Concentration: Lot specific

Buffer: Lyophilized from 5 mg BSA, 0.9 mg sodium chloride, 0.2 mg sodium phosphate, 0.05 mg Thimerosal, 0.05 mg sodium azide

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A / Topoisomerase II alpha

Background: Name/Gene ID: TOP2A
Family: Topoisomerase

Synonyms: TOP2A, DNA topoisomerase II, 170 kD, TOP2, DNA gyrase, DNA topoisomerase 2-alpha, TP2A

Gene ID: 7153

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases