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Keratin 6 antibody

This anti-Keratin 6 antibody is a Mouse Monoclonal antibody detecting Keratin 6 in IHC, IHC (p), WB and IHC (fro). Suitable for Human and Mouse.
Catalog No. ABIN599805

Quick Overview for Keratin 6 antibody (ABIN599805)

Target

Keratin 6 (KRT6)

Reactivity

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Human, Mouse

Host

  • 17
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Mouse

Clonality

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Monoclonal

Conjugate

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This Keratin 6 antibody is un-conjugated

Application

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Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

KS6-KA12
  • Specificity

    Recognizes human Keratin 6. Species cross-reactivity: Mouse.

    Purification

    Protein A purified

    Immunogen

    Keratin 6 of human callus cytoskeletal preparation.

    Isotype

    IgG1
  • Application Notes

    Approved: IHC, IHC-Fr (1:10), IHC-P (1:10), WB (1:10)

    Usage: Suitable for discrimination of keratinizing and non-keratinizing squamous cell carcinoma versus e. g. poorly differentiated adenocarcinoma. Applications: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: Reacts with Mr 56000 polypeptide of Keratin 6 of human squamous epithelia. Immunohistochemistry (Frozen, paraffin): 1:10 diluted with PBS using the ABC method. Incubate for 1 hour at RT, extended for paraffin sections. Fixation with formalin is recommended. Microwave treatment improves performance.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    Tris,  pH 7.4, 0.5 % BSA, 0.09 % sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Aliquot to Avoid repeated freezing and thawing.

    Storage

    4 °C,-20 °C

    Storage Comment

    May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
  • Target

    Keratin 6 (KRT6)

    Alternative Name

    KRT6 / CK6 / Cytokeratin 6
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