Keratin 6 antibody
Quick Overview for Keratin 6 antibody (ABIN599805)
Target
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Specificity
- Recognizes human Keratin 6. Species cross-reactivity: Mouse.
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Purification
- Protein A purified
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Immunogen
- Keratin 6 of human callus cytoskeletal preparation.
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Isotype
- IgG1
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Application Notes
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Approved: IHC, IHC-Fr (1:10), IHC-P (1:10), WB (1:10)
Usage: Suitable for discrimination of keratinizing and non-keratinizing squamous cell carcinoma versus e. g. poorly differentiated adenocarcinoma. Applications: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: Reacts with Mr 56000 polypeptide of Keratin 6 of human squamous epithelia. Immunohistochemistry (Frozen, paraffin): 1:10 diluted with PBS using the ABC method. Incubate for 1 hour at RT, extended for paraffin sections. Fixation with formalin is recommended. Microwave treatment improves performance. -
Comment
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- Tris, pH 7.4, 0.5 % BSA, 0.09 % sodium azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Aliquot to Avoid repeated freezing and thawing.
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Storage
- 4 °C,-20 °C
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Storage Comment
- May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
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- Keratin 6 (KRT6)
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Alternative Name
- KRT6 / CK6 / Cytokeratin 6
Target
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