Orexin antibody (AA 2-14)

Catalog No. ABIN600037

This Sheep Polyclonal antibody specifically detects Orexin in ELISA, IHC, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Pig, Horse, Rabbit, Bat, Hamster and Monkey.

Quick Overview for Orexin antibody (AA 2-14) (ABIN600037)

Target: Orexin (OX)

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Horse, Rabbit, Bat, Hamster, Monkey

Host: Sheep

Clonality: Polyclonal

Conjugate: This Orexin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Orexin Antibody

Binding Specificity: AA 2-14

Specificity: Recognizes human Orexin A.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Dog, Horse, Rabbit, Pig (100%) Panda (92%) Marmoset, Opossum, Chicken (85%).

Purification: Ammonium sulfate precipitation

Immunogen: Synthetic peptide corresponding to aa2-14 of human orexin A peptide, (KLH). Peptide sequence: PLPDCCRQKTCSC. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Dog, Horse, Rabbit, Pig (100%), Panda (92%), Marmoset, Opossum, Chicken (85%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:200 - 1:800), IHC, IHC-Fr (5 - 10 μg/mL), IHC-P (5 - 10 μg/mL)

Usage: Suitable for use in ELISA and Immunohistochemistry (frozen and paraffin). Immunohistochemistry (frozen): 5-10 μg/mL. ELISA: 1:200-1:800.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for Orexin

Target: Orexin (OX)

Alternative Name: HCRT / Orexin

Background: Name/Gene ID: HCRT

Synonyms: HCRT, PPORX, OX, Hypocretin, NRCLP1, Orexin

Gene ID: 3060

UniProt: O43612

Pathways: Synaptic Membrane, Feeding Behaviour