SMAD3 antibody (AA 417-425)

Catalog No. ABIN600211

This Rabbit Polyclonal antibody specifically detects SMAD3 in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Pig, Chicken, Zebrafish (Danio rerio), Cow and Fish.

Quick Overview for SMAD3 antibody (AA 417-425) (ABIN600211)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity: Human, Mouse, Rat, Pig, Chicken, Zebrafish (Danio rerio), Cow, Fish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SMAD3 Antibody

Binding Specificity: AA 417-425

Specificity: Recognizes human Smad3. Species cross-reactivity: mouse, pig, rat, amphibia, zebrafish, bovine, chicken.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to aa417-425 of human SMAD3.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:25000 - 1:100000), IHC, IHC-P (2.5 μg/mL), WB (1:2000 - 1:20000)

Usage: Suitable for use in ELISA, Immunohistochemistry (paraffin), and Western Blot. Immunohistochemistry (paraffin): 2.5 μg/mL. ELISA: 1:25000-1:100000. Western Blot: 1:2000-1:20000.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: SMAD3

Background: Name/Gene ID: SMAD3

Synonyms: SMAD3, HSPC193, HMAD-3, HsT17436, LDS1C, LDS3, Mad homolog JV15-2, MAD3, MADH3, Mothers against DPP homolog 3, JV15-2, SMA- and MAD-related protein 3, SMAD family member 3, MAD homolog 3, HSMAD3, Mad protein homolog, SMAD 3

Gene ID: 4088

UniProt: P84022

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy