PRKAA1 antibody

Catalog No. ABIN600430

This Rabbit Polyclonal antibody specifically detects PRKAA1 in WB, IHC and IF. It exhibits reactivity toward Human, Mouse, Rat, Dog, Rabbit, Chicken, Cow, Horse, Monkey, Pig, Zebrafish (Danio rerio) and Xenopus laevis.

Quick Overview for PRKAA1 antibody (ABIN600430)

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Chicken, Cow, Horse, Monkey, Pig, Zebrafish (Danio rerio), Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PRKAA1 Antibody

Specificity: Recognizes human AMPK1. Species cross-reactivity: mouse, rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic non-phosphopeptide corresponding to human AMPK1 around the phosphorylation site of threonine 174 (L-R-TP-S-C).

Type of Immunogen: Synthetic Non-phosphopeptide

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:100 - 1:200), IHC (1:50 - 1:100), WB (1:500 - 1:1000)

Usage: Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Immunofluorescence: 1:100-1:200. Western Blot: 1:500-1:1000. Immunohistochemistry: 1:50-1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS (without Mg2+, Ca2+),  pH 7.4, 150 mM sodium chloride, 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for PRKAA1

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Alternative Name: PRKAA1 / AMPK Alpha 1

Background: Name/Gene ID: PRKAA1
Subfamily: AMPK/SNF1
Family: Protein Kinase

Synonyms: PRKAA1, ACACA kinase, AMPK alpha 1, AMPK alpha-1 chain, Acetyl-CoA carboxylase kinase, AMPKa1, AMPK subunit alpha-1, AMPK, AMPK1, Tau-protein kinase PRKAA1, HMGCR kinase

Gene ID: 5562

UniProt: Q13131

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect