ATG5 antibody (AA 1-50) (HRP)

Catalog No. ABIN600461

The Rabbit Polyclonal anti-ATG5 antibody has been validated for WB and IHC. It is suitable to detect ATG5 in samples from Human, Mouse, Rat, Cow, Monkey, Pig and Xenopus laevis.

Quick Overview for ATG5 antibody (AA 1-50) (HRP) (ABIN600461)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human, Mouse, Rat, Cow, Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG5 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ATG5 Antibody (HRP)

Binding Specificity: AA 1-50

Specificity: Selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing aa1-79. The calpain cleaved form of ATG5 is missing aa195-275. Cellular Localization: Cytoplasm. Co-localizes with non-muscle actin. Species cross-reactivity: human, mouse, rat, bovine, porcine, primate, Xenopus and Zebrafish. MW of human antigen: 33kD.

Purification: Immunoaffinity purified

Immunogen: A synthetic peptide made to an N-terminal region (aa1-50) of the human ATG5 protein.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC (2.5 μg/mL), WB (1:500)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:500. Immunohistochemistry: 2.5 μg/mL. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide, 30 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do Not Freeze! Aliquots are stable for at least 3 months. Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: APG5 / ATG5

Background: Name/Gene ID: ATG5

Synonyms: ATG5, APG5, APG5-LIKE, APG5L, Apoptosis specific protein, Apoptosis-specific protein, Autophagy related 5, HAPG5, ASP, Autophagy protein 5

Gene ID: 9474

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy