ATG5 antibody (AA 1-50) (Biotin)

Catalog No. ABIN600462

This anti-ATG5 antibody is a Rabbit Polyclonal antibody detecting ATG5 in WB and IHC. Suitable for Human, Mouse, Rat, Cow, Monkey, Pig and Xenopus laevis.

Quick Overview for ATG5 antibody (AA 1-50) (Biotin) (ABIN600462)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human, Mouse, Rat, Cow, Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG5 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ATG5 Antibody (Biotin)

Binding Specificity: AA 1-50

Specificity: Selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing aa1-79. The calpain cleaved form of ATG5 is missing aa195-275. Cellular Localization: Cytoplasm. Co-localizes with non-muscle actin. Species cross-reactivity: human, mouse, rat, bovine, porcine, primate, Xenopus and Zebrafish. MW of human antigen: 33kD.

Purification: Immunoaffinity purified

Immunogen: A synthetic peptide made to an N-terminal region (aa1-50) of the human ATG5 protein.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC (2.5 μg/mL), WB (1:500)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:500. Immunohistochemistry: 2.5 μg/mL. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide, 30 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C.

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: APG5 / ATG5

Background: Name/Gene ID: ATG5

Synonyms: ATG5, APG5, APG5-LIKE, APG5L, Apoptosis specific protein, Apoptosis-specific protein, Autophagy related 5, HAPG5, ASP, Autophagy protein 5

Gene ID: 9474

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy