CTNNB1 antibody (AA 36-44)

Catalog No. ABIN600589

This Mouse Monoclonal antibody specifically detects CTNNB1 in WB, IHC, ELISA and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CTNNB1 antibody (AA 36-44) (ABIN600589)

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTNNB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Product Details anti-CTNNB1 Antibody

Binding Specificity: AA 36-44

Specificity: Specific for the active form of beta-catenin, non-phosphorylated on Ser37 and Thr41.1 A non-specific protein, Mr 130kD, was also detected when used at higher concentrations. Species cross-reactivity: Mouse and rat (100 % ). Predicted to cross-react with frog and chicken (97/100), porcine (99/100) and zebrafish (91/100) based on epitope sequence homology. Does not cross-react with Drosophila. Others not tested.

Purification: Protein G purified

Immunogen: Fusion protein corresponding to residues 1-100 of human beta-catenin with a C-terminal histidine tag. Epitope: Corresponds to aa 36-44 (HSGATTTAP) of human beta-catenin, Mr 92kD.

Type of Immunogen: Fusion protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, ICC, IHC, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects beta-catenin in RIPA lysates from A431 cells. Immunohistochemistry: Detects b-catenin in mouse embryo sections. Immunocytochemistry: Detects b-catenin in LiCl-treated 293Tcells fixed with methanol.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4 No stabilizing proteins added. No preservative added. Also available w/ azide. See C2069-47

Preservative: Sodium azide, Without preservative

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 1 year at -20°C.

Target Details for CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Alternative Name: CTNNB1 / Beta Catenin

Background: Name/Gene ID: CTNNB1
Family: Catenin

Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin

Gene ID: 1499

UniProt: P35222

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2