CDC25A antibody (pSer75)

Catalog No. ABIN600985

This Rabbit Polyclonal antibody specifically detects CDC25A in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse and Rabbit.

Quick Overview for CDC25A antibody (pSer75) (ABIN600985)

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CDC25A Antibody

Binding Specificity: pSer75

Specificity: Recognizes human cdc25A when phosphorylated at serine 75. Species cross-Reactivity: mouse, rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide corresponding to human cdc25A around the phosphorylation site of serine 75 (M-G-SP-S-E).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC (1:50 - 1:100), WB (1:500 - 1:1000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot:1:500-1:1000. Immunohistochemistry:1:50-1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS (without Mg2+, Ca2+),  pH 7.4, 150 mM sodium chloride, 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: CDC25A

Background: Name/Gene ID: CDC25A
Subfamily: CDC25
Family: Protein Phosphatase

Synonyms: CDC25A, CDC25A2-CAG isoform, Cell division cycle 25A, CDC25A phosphatase, CDC25Hu1, M-phase inducer phosphatase 1, CDC25A2

Gene ID: 993

UniProt: P30304

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase