Cyclin B1 antibody (pSer147)

Catalog No. ABIN601130

The Rabbit Polyclonal anti-Cyclin B1 antibody has been validated for IHC and IF. It is suitable to detect Cyclin B1 in samples from Human, Mouse, Rat, Hamster, Cow, Dog, Rabbit, Pig, Horse, Monkey, Xenopus laevis and Zebrafish (Danio rerio).

Quick Overview for Cyclin B1 antibody (pSer147) (ABIN601130)

Target: Cyclin B1 (CCNB1)

Reactivity: Human, Mouse, Rat, Hamster, Cow, Dog, Rabbit, Pig, Horse, Monkey, Xenopus laevis, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Cyclin B1 Antibody

Binding Specificity: pSer147

Specificity: Recognizes human Cyclin B1 when phosphorylated at serine 147.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide corresponding to human Cyclin B1 around the phosphorylation site of Serine 147 (A-F-SP-D-V).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:100 - 1:200), IHC (1:50 - 1:100)

Usage: Suitable for use in Immunofluorescence and Immunohistochemistry. Immunofluorescence: 1:100-1:200. Immunohistochemistry: 1:50-100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS (without Mg2+, Ca2+),  pH 7.4, 150 mM sodium chloride, 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: CCNB1 / Cyclin B1

Background: Name/Gene ID: CCNB1

Synonyms: CCNB1, CCNB, Cyclin B1, G2/mitotic-specific cyclin-B1, G2/mitotic-specific cyclin B1

Gene ID: 891

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase