IL-4 antibody

Catalog No. ABIN601663

This Rat Monoclonal antibody specifically detects IL-4 in WB, ELISA, IHC, FACS and IHC (fro). It exhibits reactivity toward Human and Monkey.

Quick Overview for IL-4 antibody (ABIN601663)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity: Human, Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-IL-4 Antibody

Specificity: Recognizes human Interleukin - 4 (IL-4), a 20kD cytokine which is expressed by a subpopulation of activated T cells and mast cells. Reported to neutralize IL-4 activity (2,5). Species cross-reactivity: Rhesus Monkey.

Purification: Protein G purified

Immunogen: Purified CHO cell expressed recombinant human IL-4.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo (1:10 - 1:50), IHC, IHC-Fr, WB (1:500 - 1:1000)

Usage: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Western Blot. Flow Cytometry: 1:10-1:50, 10 μL labels 10^6 cells in 100 μL. *Membrane permeabilization is required for this application. Western Blot: 1:500-1:1000. Immunohistochemistry: frozen.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL4

Background: Name/Gene ID: IL4
Family: Hormone

Synonyms: IL4, B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, BCGF1, BSF-1, Binetrakin, BCGF-1, Interleukin 4, Interleukin-4, Pitrakinra, BSF1, IL-4

Gene ID: 3565

UniProt: P05112

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation