Muscarinic Acetylcholine Receptor M2 antibody (AA 168-192)

Catalog No. ABIN601952

The Mouse Monoclonal anti-Muscarinic Acetylcholine Receptor M2 antibody has been validated for WB, ELISA, IHC, IF and IHC (fro). It is suitable to detect Muscarinic Acetylcholine Receptor M2 in samples from Human, Mouse, Rat, Monkey, Cow, Dog, Guinea Pig, Pig, Sheep and Horse.

Quick Overview for Muscarinic Acetylcholine Receptor M2 antibody (AA 168-192) (ABIN601952)

Target: Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))

Reactivity: Human, Mouse, Rat, Monkey, Cow, Dog, Guinea Pig, Pig, Sheep, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Muscarinic Acetylcholine Receptor M2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B8E5

Product Details anti-Muscarinic Acetylcholine Receptor M2 Antibody

Binding Specificity: AA 168-192

Specificity: Recognizes the pentapeptide VRTVE (aa 168-172) corresponding to the N-terminal part of the second extracellular loop of the human M2 receptor. Species cross-reactivity: rat, mouse and guinea pig.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Sheep, Panda, Dog, Bovine, Horse, Pig, Guinea pig (100%) Elephant, Bat, Rabbit (96%) Hamster, Opossum, Platypus (92%) Xenopus (88%) Stickleback, Pufferfish, Zebrafish (84%) Turkey, Chicken (80%).

Purification: Protein G purified

Immunogen: Synthetic peptide corresponding to the second extracellular loop aa168-192, VRTVEDGECYIQFFSNAAVTFGTAI(C) of human muscarinic acetylcholine receptor M2. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Sheep, Panda, Dog, Bovine, Horse, Pig, Guinea pig (100%), Elephant, Bat, Rabbit (96%), Hamster, Opossum, Platypus (92%), Xenopus (88%), Stickleback, Pufferfish, Zebrafish (84%), Turkey, Chicken (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (1:50), IF (1:50), IHC, IHC-Fr (1:50), WB (1:50)

Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. ELISA: 1:50. Western Blot: 1:50 under non-reducing conditions. Immunohistochemistry (frozen): 1:50. Immunofluorescence: 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % BSA

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for Muscarinic Acetylcholine Receptor M2

Target: Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))

Alternative Name: CHRM2 / M2

Background: Name/Gene ID: CHRM2
Subfamily: Acetylcholine
Family: GPCR

Synonyms: CHRM2, 7TM receptor, HM2, M2 muscarinic receptor, M2-mAChR, Muscarinic receptor M2, Muscarinic M2 receptor

Gene ID: 1129

UniProt: P08172