MYOD1 antibody (AA 77-215)

Catalog No. ABIN601982

The Mouse Monoclonal anti-MYOD1 antibody has been validated for IHC. It is suitable to detect MYOD1 in samples from Human.

Quick Overview for MYOD1 antibody (AA 77-215) (ABIN601982)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYOD1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-MYOD1 Antibody

Binding Specificity: AA 77-215

Specificity: Recognizes human MyoD.

Purification: Protein G purified

Sterility: 0.2 μm filtered

Immunogen: Recombinant human MyoD, aa 77-215 (E. coli). Accession# (P15172).

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: IHC (10 μg/mL)

Usage: Immunohistochemistry:10 μg/mL to detect MyoD in C2C12 mouse muscle myoblast cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with. PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for. 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4 °C followed by fluorochrome-coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile 40-50 % glycerol, PBS.

Concentration: Lot specific

Buffer: Lyophilized from 0.2 um filtered solution in PBS, 5 % trehalose

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Alternative Name: MYOD / MYOD1

Background: Name/Gene ID: MYOD1

Synonyms: MYOD1, BHLHc1, Myogenic differentiation 1, Myf-3, MYOD, Myogenic factor 3, PUM, MYF3

Gene ID: 4654

UniProt: P15172

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development