PTK2B antibody (pTyr402)

Catalog No. ABIN602219

The Rabbit Polyclonal anti-PTK2B antibody is suitable to detect PTK2B in samples from Human, Mouse, Rat, Dog, Rabbit, Monkey, Pig, Horse, Cow and Xenopus laevis. It has been validated for WB and IHC.

Quick Overview for PTK2B antibody (pTyr402) (ABIN602219)

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Monkey, Pig, Horse, Cow, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTK2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PTK2B Antibody

Binding Specificity: pTyr402

Specificity: Recognizes human Pyk2 when phosphorylated at tyrosine 402. Species cross-reactivity: mouse, rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide corresponding to human Pyk2 around the phosphorylation site of tyrosine 402 (D-I-YP-A-E).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC (1:50 - 1:100), WB (1:500 - 1:1000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:500-1:1000. Immunohistochemistry: 1:50-1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS (without Mg2+, Ca2+),  pH 7.4, 150 mM sodium chloride, 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for PTK2B

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Alternative Name: PTK2B / PYK2

Background: Name/Gene ID: PTK2B
Subfamily: Focal Adhesion Kinase
Family: Protein Kinase

Synonyms: PTK2B, CADTK, CAKB, Cell adhesion kinase beta, FADK 2, FADK2, Focal adhesion kinase 2, FRNK, FAK2, Proline-rich tyrosine kinase 2, Protein-tyrosine kinase 2-beta, Protein kinase B, Protein tyrosine kinase 2 beta, PYK2, RAFTK, CAK-beta, PKB, PTK

Gene ID: 2185

UniProt: Q14289

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling