Src antibody (pTyr529)

Catalog No. ABIN602330

The Rabbit Polyclonal anti-Src antibody has been validated for WB and IHC. It is suitable to detect Src in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Chicken, Cow, Pig, Dog, Monkey, Horse and Xenopus laevis.

Quick Overview for Src antibody (pTyr529) (ABIN602330)

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Chicken, Cow, Pig, Dog, Monkey, Horse, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Src antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Src Antibody

Binding Specificity: pTyr529

Specificity: Recognizes human Src when phosphorylated at Tyr529. Species cross-Reactivity: mouse, rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide corresponding to human Src around the phosphorylation site of tyrosine 529 (P-Q-YP-Q-P).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC (1:50 - 1:100), WB (1:500 - 1:1000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot:1:500-1:1000. Immunohistochemistry:1:50-1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS (without Mg2+, Ca2+),  pH 7.4, 150 mM sodium chloride, 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for Src

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Alternative Name: SRC

Background: Name/Gene ID: SRC
Subfamily: Src
Family: Protein Kinase

Synonyms: SRC, ASV, Pp60c-src, Tyrosine kinase pp60c-src, Tyrosine-protein kinase SRC-1, v-src, C-SRC, p60-Src, Proto-oncogene c-Src, SRC1

Gene ID: 6714

UniProt: P12931

Pathways: JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling