ZAP70 antibody

Catalog No. ABIN602570

The Rabbit Polyclonal anti-ZAP70 antibody has been validated for WB and IHC. It is suitable to detect ZAP70 in samples from Human, Mouse, Rat, Cow, Rabbit, Chicken, Horse, Monkey and Xenopus laevis.

Quick Overview for ZAP70 antibody (ABIN602570)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Chicken, Horse, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ZAP70 Antibody

Specificity: Recognizes human ZAP 70. Species cross-Reactivity: mouse, rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic non-phosphopeptide corresponding to human Zap-70 around the phosphorylation site of tyrosine 319 (S-P-YP-S-D).

Type of Immunogen: Synthetic Non-phosphopeptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC (1:50 - 1:100), WB (1:500 - 1:1000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot:1:500-1:1000. Immunohistochemistry:1:50-1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS (without Mg2+, Ca2+),  pH 7.4, 150 mM sodium chloride, 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70

Background: Name/Gene ID: ZAP70
Subfamily: Syk/ZAP70
Family: Protein Kinase

Synonyms: ZAP70, 70 kDa zeta-associated protein, SRK, STCD, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70, TZK, ZAP-70, Zeta-associated protein 70

Gene ID: 7535

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway