ADAM10 antibody (APC)

Catalog No. ABIN602624

Product Details anti-ADAM10 Antibody (APC)

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ADAM10 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Specificity: Recognizes human ADAM10 (APC).

Purification: Purified

Immunogen: Mouse myeloma cell line NS0-derived recombinant human ADAM10 (aa Thr214 - Glu672, UniProt Accession #O14672).

Clone: 163003

Isotype: IgG2b

Application Details

Application Notes: Approved: Flo

Usage: Suitable for use in Flow Cytometry Flow Cytometry: Intracellular staining antibodies are designed for multiparameter flow cytometric analysis of cells. To stain for surface proteins (e. g. CD3, CD4, CD8) in addition to the intracellular protein, we recommend that the investigator determine whether the fixation and permeabilization steps adversely affect the surface protein. If so, surface staining of cells prior to fixation and permeabilization is recommended. For intracellular staining, cells must first be fixed and permeabilized. Use of 4 % paraformaldehyde in PBS as a fixative is recommended. Other formulations or tissue fixatives may affect the staining properties of the monoclonal antibody. For permeabilization, 0.1 % saponin in a balanced salt solution is effective in facilitating antibody entry into cells. Due to the reversible nature of cell membrane permeabilization, saponin must be included in all buffers used (i. e. both the staining and washing steps). 10 μL of undiluted product can be used to stain 1 x 10e6 cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 40-50 % glycerol, PBS.

Concentration: Lot specific

Buffer: Lyophilized from 25 μg antibody, 1 mL PBS, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C.

Target Details for ADAM10

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Alternative Name: ADAM10 (ADAM10 Products)

Background: Name/Gene ID: ADAM10
Subfamily: Metallopeptidase M12B
Family: Protease

Synonyms: ADAM10, AD10, ADAM 10, Kuzbanian, HsT18717, CD156c, CD156c antigen, CDw156, Kuz, Kuzbanian protein homolog, MADM

Gene ID: 102

UniProt: O14672

Pathways: Notch Signaling, EGFR Signaling Pathway