AGRP antibody (Biotin)

Catalog No. ABIN602716

This Goat Polyclonal antibody specifically detects AGRP in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for AGRP antibody (Biotin) (ABIN602716)

Target: AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AGRP antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-AGRP Antibody (Biotin)

Specificity: Recognizes human AgRP. ~40 % cross-reactivity with rmAgRP.

Purification: Immunoaffinity purified

Sterility: Sterile filtered

Immunogen: Sf 21-derived, recombinant human Agouti-Related Protein.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western Blot. Using plates coated with 100 μL/well of the capture antibody, in combination with 100 μL/well of the detection antibody at 50 ng/mL, an ELISA for sample volumes of 100 μL can be obtained. To arrive at the optimal dose range for. this ELISA, set up a two-fold dilution series of the protein standard stAgRPing with 4 ng/mL. Western Blot: 0.1-0.2 μg/mL. The detection limit for rhAgRP is approximately 1 ng/lane under non-reducing and reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile TBS,  pH 7.3, 0.1 % BSA.

Concentration: Lot specific

Buffer: Lyophilized from 0.2 um sterile-filtered solution in PBS, 50 μg BSA

Storage: 4 °C,-20 °C

Storage Comment: Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

Target Details for AGRP

Target: AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))

Alternative Name: AGRP

Background: Name/Gene ID: AGRP

Synonyms: AGRP, Agouti (mouse) related protein, Agouti-related protein, Agouti-related transcript, AGRT, ASIP2

Gene ID: 181

UniProt: O00253

Pathways: Feeding Behaviour, Photoperiodism