FAP antibody (AA 26-760)

Catalog No. ABIN603200

The Mouse Monoclonal anti-FAP antibody has been validated for ELISA and FACS. It is suitable to detect FAP in samples from Human.

Quick Overview for FAP antibody (AA 26-760) (ABIN603200)

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAP antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Clone: 9H122

Product Details anti-FAP Antibody

Binding Specificity: AA 26-760

Specificity: Recognizes human Fibroblast Activation Protein alpha. Does not cross-react with recombinant human DPP6.

Purification: Immunoaffinity purified

Immunogen: Purified, Sf 21-derived, recombinant human FAP (rhFAP, aa 26-760, Accession# Q12884).

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (2 - 8 μg/mL), Flo (25 μg/mL)

Usage: Suitable for use in Flow Cytometry and ELISA. Flow Cytometry: 25 μg/mL. 10 μL of the diluted solution labels 1-2.5x10^5 WI-38 cells in a total reaction volume 200 μL. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as an IgG (FITC) anti-mouse. ELISA: 2-8 μg/mL. Can be used in combination with biotinylated human FAP detection antibody and recombinant human FAP as the standard.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 5 % trehalose.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for FAP

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Alternative Name: FAP alpha

Background: Name/Gene ID: FAP
Subfamily: Serine S9
Family: Protease

Synonyms: FAP, DPPIV, FAP alpha, Fibroblast activation protein, FAPA, Seprase

Gene ID: 2191

UniProt: Q12884

Pathways: Tube Formation