FOLR1 antibody (AA 25-233)

Catalog No. ABIN603215

The Mouse Monoclonal anti-FOLR1 antibody has been validated for WB, ELISA, FACS and ICC. It is suitable to detect FOLR1 in samples from Human.

Quick Overview for FOLR1 antibody (AA 25-233) (ABIN603215)

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOLR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-FOLR1 Antibody

Binding Specificity: AA 25-233

Specificity: Recognizes human FOLR1. In Direct ELISA, shows no cross-reactivity with recombinant human FOLR2, 3 or 4. Species sequence Homology: mouse, 83 % .

Purification: Protein G purified

Immunogen: Purified, CHO-derived, recombinant human FOLR1 (rhFOLR1, aa 25-233, Accession# P15328).

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), ICC, WB

Usage: ELISA (Capture): 2-8 μg/mLFlow Cytometry: 2.5 μg labels 10e6 cells. Tested in MCF-7 cells.Western Blot: 2 μg/mL detects human FOLR1 in human cortex tissue and HeLa cells under non-reducing conditions only. Immunocytochemistry: 8-25 μg/mL. Tested on immersion fixed MCF-7 cells.Optimal dilutions to be determined by the researcher.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 200 μL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for FOLR1

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Alternative Name: FOLR1 / Folate Receptor Alpha

Background: Name/Gene ID: FOLR1

Synonyms: FOLR1, Adult folate-binding protein, FBP, Folate receptor alpha, KB cells FBP, Folate binding protein, Folate receptor 1, Folate receptor 1 (adult), Folate receptor, adult, FOLR, FR-alpha

Gene ID: 2348

UniProt: P15328

Pathways: Dicarboxylic Acid Transport