IDUA antibody (AA 26-653)

Catalog No. ABIN603316

This Mouse Monoclonal antibody specifically detects IDUA in ELISA and WB. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for IDUA antibody (AA 26-653) (ABIN603316)

Target: IDUA (Iduronidase, alpha-L- (IDUA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IDUA antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Clone: 452619

Product Details anti-IDUA Antibody

Binding Specificity: AA 26-653

Specificity: Recognizes human Alpha-L-Iduronidase. Species sequence Homology: Mouse (79 % ), chicken (61 %).

Purification: Protein G purified

Immunogen: NS0-derived, recombinant human IDUA aa26-653.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. Direct ELISA: 0.5-1 μg/mL. The detection limit is ~1 ng/well. Antibody was tested using full-length, recombinant protein. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit is ~5 ng/lane under non-reducing and reducing conditions. Antibody tested in WB using the full-length recombinant protein. Chemiluminescent detection will increase sensitivity by 5-50 fold.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

References for anti-IDUA antibody (ABIN603316)

Gutierrez-Valdes, Häkkinen, Lemasson, de Groot, Ele-Ekouna, Guillet, Cardon, Ritala: "Improving yield of a recombinant biologic in a Brassica hairy root manufacturing process." in: Biotechnology and bioengineering, (2022) (PubMed).

Cardon, Pallisse, Bardor, Caron, Vanier, Ele Ekouna, Lerouge, Boitel-Conti, Guillet: "Brassica rapa hairy root based expression system leads to the production of highly homogenous and reproducible profiles of recombinant human alpha-L-iduronidase." in: Plant biotechnology journal, Vol. 17, Issue 2, pp. 505-516, (2019) (PubMed).

Target Details for IDUA

Target: IDUA (Iduronidase, alpha-L- (IDUA))

Alternative Name: IDUA / MPS1

Background: Name/Gene ID: IDUA

Synonyms: IDUA, Alpha-L-iduronidase, MPS1, Iduronidase, alpha-L-, IDA

Gene ID: 3425

Pathways: Glycosaminoglycan Metabolic Process