ATG12 antibody (Ser36)

Catalog No. ABIN604281

The Rabbit Monoclonal anti-ATG12 antibody (ABIN604281) specifically detects ATG12 in WB and IP. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for ATG12 antibody (Ser36) (ABIN604281)

Target: ATG12 (Autophagy Related 12 (ATG12))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ATG12 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-ATG12 Antibody

Binding Specificity: Ser36

Specificity: Recognizes human endogenous levels of total free and Atg5 bound Atg12 protein. Species cross-reactivity: mouse, rat, monkey.

Immunogen: Synthetic peptide corresponding to residues surrounding Ser36 of human Atg12 protein (KLH- coupled).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IP (1:100), WB (1:1000)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5 % w/v BSA, 1X TBS, 0.1 %. Tween-20 at 4 °C with gentle shaking, overnight. Immunoprecipitation: 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least1 year at -20°C.

Target Details for ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Alternative Name: APG12 / ATG12

Background: Name/Gene ID: ATG12

Synonyms: ATG12, Autophagy-related protein 12, APG12L, Autophagy related 12, FBR93, APG12, APG12-like, Ubiquitin-like protein ATG12, HAPG12

Gene ID: 9140

UniProt: O94817

Pathways: Autophagy