Vinculin antibody

Catalog No. ABIN604849

This Mouse Monoclonal antibody specifically detects Vinculin in WB, IHC, IF, IP and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Vinculin antibody (ABIN604849)

Target: Vinculin (VCL)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Vinculin Antibody

Brand: IHC-plus™

Specificity: Recognizes human vinculin.

Purification: Protein G purified

Immunogen: Semi-purified vinculin. MW of Antigen: 130kD (vinculin) and 150kD (meta-vinculin). Cellular Localization: Cell membrane and cytoplasmic.

Type of Immunogen: Protein extract

Isotype: IgG1

Application Details

Application Notes: Approved: IF, IHC, IHC-P (2.5 - 5 μg/mL), IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Studies of vinculin in platelet activation. Immunoprecipitation (Denatured): 2 μg/mg protein lysate (Use Protein G). Western Blot: 1-2 μg/mL for 2hrs at RT. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4. No preservatives or stabilizing proteins added.

Preservative: Azide free

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: VCL / Vinculin

Background: Name/Gene ID: VCL
Family: Cytoskeleton

Synonyms: VCL, CMD1W, CMH15, Metavinculin, Vinculin, MVCL

Gene ID: 7414

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2