CD19 antibody (AA 540-557)

Catalog No. ABIN609293

The Mouse Monoclonal anti-CD19 antibody has been validated for FACS, WB, IHC, IP, ELISA and IHC (p). It is suitable to detect CD19 in samples from Human.

Quick Overview for CD19 antibody (AA 540-557) (ABIN609293)

Target: CD19 (CD19 Molecule (CD19))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD19 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD19 Antibody

Binding Specificity: AA 540-557

Specificity: Recognizes the C-terminal cytoplasmic tail sequence (CGPDPAWGGGGRMGTWSTR) of human CD19, a 95kD cell surface glycoprotein, which is expressed by cells of the B cell lineage and follicular dendritic cells. CD19 is absent on plasma cells. Detects a band of ~95kD in Raji cell lysates under reducing conditions.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon, Monkey (94%) Elephant, Panda, Dog (89%) Pig (83%).

Purification: Protein G purified

Immunogen: CD19 peptide C-GPDPAWGGGGRMGTWSTR (C-terminus) coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon, Monkey (94%), Elephant, Panda, Dog (89%), Pig (83%).

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo (1:100 - 1:200), IHC, IHC-P (1:100 - 1:200), IP, WB (1:100 - 1:200)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry (paraffin), Immunoprecipitation, and Flow Cytometry. Flow Cytometry: 1:100-1:200, 10 μL labels 10e6 cells in 100 μLImmunohistochemistry (paraffin): 1:100-1:200. Membrane permeabilization and antigen retrieval using heat treatment methods prior to staining of paraffin sections.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19

Background: Name/Gene ID: CD19

Synonyms: CD19, B-lymphocyte antigen CD19, CD19 Molecule, Differentiation antigen CD19, T-cell surface antigen Leu-12, B4, CD19 antigen, CVID3

Gene ID: 930

UniProt: P15391

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway