CD35 antibody

Catalog No. ABIN7472905

The Mouse Monoclonal anti-CD35 antibody has been validated for FACS, IHC, WB, IHC (p) and IHC (fro). It is suitable to detect CD35 in samples from Human, Baboon, Monkey and Cynomolgus.

Quick Overview for CD35 antibody (ABIN7472905)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivity: Human, Baboon, Monkey, Cynomolgus

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD35 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CD35 Antibody

Specificity: Recognizes human CD35. Species cross-reactivity: Cynomolgus monkey, Rhesus Monkey, Baboon.

Purification: Protein A purified

Immunogen: Human acute monocytic leukemia cells.

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:50 - 1:200), IHC, IHC-Fr (1 - 10 μg/mL), IHC-P (1 - 10 μg/mL), WB (1:50 - 1:200)

Usage: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen and paraffin), and Western Blot. Flow Cytometry: 1:50-1:200, 10 μL labels 10^6 cells in 100 μL. Immunohistochemistry (frozen): 1-10 μg/mL. Immunohistochemistry (paraffin): 1-10 μg/mL. Requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: CR1 / CD35

Background: Name/Gene ID: CR1
Subfamily: Serine S1
Family: Protease

Synonyms: CR1, C3b/C4b receptor, C3-binding protein, CD35, C3BR, Knops blood group antigen, C4BR, CD35 antigen, Complement receptor type 1, KN

Gene ID: 1378

UniProt: P17927

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity