RAF1 antibody (pSer259)

Catalog No. ABIN6136222

This anti-RAF1 antibody is a Rabbit Polyclonal antibody detecting RAF1 in WB and IP. Suitable for Human.

Quick Overview for RAF1 antibody (pSer259) (ABIN6136222)

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-RAF1 Antibody

Binding Specificity: pSer259

Sequence: STSTP

Cross-Reactivity: Human, Mouse

Characteristics: Phosphorylated Antibodies

Immunogen: A synthetic phosphorylated peptide around S259 of human Phospho-Raf1-S259 (NP_002871.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IP,1:50 - 1:100

Comment:

HIGH QUALITY

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for RAF1

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Alternative Name: RAF1

Background: This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.,CMD1NN,CRAF,NS5,Raf-1,c-Raf,RAF1,Cancer,Signal Transduction,G protein signaling,G2/M DNA Damage Checkpoint,Kinase,Serine/threonine kinases,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology & Developmental Biology,Apoptosis,Inhibition of Apoptosis,Growth factor,ESC Pluripotency and Differentiation,Endocrine & Metabolism,Mitochondrial metabolism,Mitochondrial markers,Insulin Receptor Signaling Pathway,Immunology & Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,IL-6 Receptor Signaling Pathway,Neuroscience,Protein phosphorylation,RAF1

Molecular Weight: 73 kDa/75 kDa

Gene ID: 5894

UniProt: P04049

Pathways: MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling