DFFB antibody (AA 109-338)

Catalog No. ABIN6139552

This anti-DFFB antibody is a Rabbit Polyclonal antibody detecting DFFB in WB and IHC. Suitable for Human.

Quick Overview for DFFB antibody (AA 109-338) (ABIN6139552)

Target: DFFB (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DFFB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-DFFB Antibody

Binding Specificity: AA 109-338

Sequence: DEQAPQRQRL LADLLHNVSQ NIAAETRAED PPWFEGLESR FQSKSGYLRY SCESRIRSYL REVSSYPSTV GAEAQEEFLR VLGSMCQRLR SMQYNGSYFD RGAKGGSRLC TPEGWFSCQG PFDMDSCLSR HSINPYSNRE SRILFSTWNL DHIIEKKRTI IPTLVEAIKE QDGREVDWEY FYGLLFTSEN LKLVHIVCHK KTTHKLNCDP SRIYKPQTRL KRKQPVRKRQ

Cross-Reactivity: Human, Mouse

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 109-338 of human DFFB (NP_004393.1).

Isotype: IgG

Application Details

Application Notes: WB,1:200 - 1:1000,IHC,1:50 - 1:200

Comment:

HIGH QUALITY

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for DFFB

Target: DFFB (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB))

Alternative Name: DFFB

Background: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined.,DFFB,CAD,CPAN,DFF-40,DFF2,DFF40,Epigenetics & Nuclear Signaling,Cell Biology & Developmental Biology,Apoptosis,Cell Cycle,Death Receptor Signaling Pathway,Endocrine & Metabolism,DFFB

Molecular Weight: 11 kDa/12 kDa/30 kDa/39 kDa

Gene ID: 1677

UniProt: O76075

Pathways: Apoptosis, Caspase Cascade in Apoptosis