FZR1 antibody (AA 224-493)

Catalog No. ABIN6140852

The Rabbit Polyclonal anti-FZR1 antibody has been validated for WB and IF. It is suitable to detect FZR1 in samples from Human.

Quick Overview for FZR1 antibody (AA 224-493) (ABIN6140852)

Target: FZR1 (Fizzy/cell Division Cycle 20 Related 1 (FZR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-FZR1 Antibody

Binding Specificity: AA 224-493

Sequence: DLSVEGDSVT SVGWSERGNL VAVGTHKGFV QIWDAAAGKK LSMLEGHTAR VGALAWNAEQ LSSGSRDRMI LQRDIRTPPL QSERRLQGHR QEVCGLKWST DHQLLASGGN DNKLLVWNHS SLSPVQQYTE HLAAVKAIAW SPHQHGLLAS GGGTADRCIR FWNTLTGQPL QCIDTGSQVC NLAWSKHANE LVSTHGYSQN QILVWKYPSL TQVAKLTGHS YRVLYLAMSP DGEAIVTGAG DETLRFWNVF SKTRSTKESV SVLNLFTRIR

Cross-Reactivity: Human, Mouse

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 224-493 of human FZR1 (NP_057347.2).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IF,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for FZR1

Target: FZR1 (Fizzy/cell Division Cycle 20 Related 1 (FZR1))

Alternative Name: FZR1

Background: Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Acts as an adapter for APC/C to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, may play a role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ over error-free, RBBP8-mediated homologous recombination (HR.,FZR1,CDC20C,CDH1,FZR,FZR2,HCDH,HCDH1,Cell Biology & Developmental Biology,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,FZR1

Molecular Weight: 44 kDa/54 kDa/55 kDa

Gene ID: 51343

UniProt: Q9UM11

Pathways: DNA Replication, Synthesis of DNA