Caspase 7 antibody

Catalog No. ABIN614356

This Mouse Monoclonal antibody specifically detects Caspase 7 in WB and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Monkey, Pig, Dog, Rabbit, Hamster and Sheep.

Quick Overview for Caspase 7 antibody (ABIN614356)

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Reactivity: Human, Mouse, Rat, Monkey, Pig, Dog, Rabbit, Hamster, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 07-01-11

Product Details anti-Caspase 7 Antibody

Specificity: Detects an ~37 kDa protein, corresponding to the apparent molecular mass of the Caspase-7 on SDS-PAGE Immunoblots, in samples from Human, Mouse, Rat, Monkey, Dog, Sheep, Rabbit, Pig and Hamster origins. This antibody recognizes the unprocessed pro-caspase-7 (p35), caspase-7 lacking the short amino- terminal pro domain (32 kDa) and the fully processed Caspase-7 (p19) in apoptotic cell extracts.

Cross-Reactivity (Details): Species reactivity (expected):Canine, Hamster, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep.
Species reactivity (tested):Human.

Purification: Protein G Chromatography.

Immunogen: Recombinant Human CASP7.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.4 mg/mL

Buffer: 10 mM Sodium Phosphate, pH 7.2, 50 mM Sodium Chloride with 0.1 mM PMSF

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Caspase 7

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Alternative Name: Caspase-7

Background: Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis, initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.Synonyms: Apoptotic protease Mch-3, CASP-7, CASP7, CMH-1, ICE-like apoptotic protease 3, MCH3

Gene ID: 840

NCBI Accession: NP_001218

UniProt: P55210

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity