METTL4 antibody (AA 1-190)

Catalog No. ABIN6143773

Product Details anti-METTL4 Antibody

Target: METTL4 (Methyltransferase Like 4 (METTL4))

Binding Specificity: AA 1-190

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This METTL4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Sequence: MSVVHQLSAG WLLDHLSFIN KINYQLHQHH EPCCRKKEFT TSVHFESLQM DSVSSSGVCA AFIASDSSTK PENDDGGNYE MFTRKFVFRP ELFDVTKPYI TPAVHKECQQ SNEKEDLMNG VKKEISISII GKKRKRCVVF NQGELDAMEY HTKIRELILD GSLQLIQEGL KSGFLYPLFE KQDKGSKPIT

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human METTL4 (NP_073751.3).

Isotype: IgG

Application Details

Application Notes: WB,1:1000 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for METTL4

Target: METTL4 (Methyltransferase Like 4 (METTL4))

Alternative Name: METTL4 (METTL4 Products)

Synonyms: HsT661 antibody, 2410198H06Rik antibody, A730091E08Rik antibody, AV296509 antibody, RGD1306451 antibody, methyltransferase like 4 antibody, METTL4 antibody, Mettl4 antibody

Background: N(6-adenine-specific methyltransferase that can methylate both RNAs and DNA. Acts as a N(6-adenine-specific RNA methyltransferase by catalyzing formation of N6,2'-O-dimethyladenosine (m6A(m on internal positions of U2 small nuclear RNA (snRNA: methylates the 6th position of adenine residues with a pre-deposited 2'-O-methylation. Internal m6A(m methylation of snRNAs regulates RNA splicing. Also able to act as a N(6-adenine-specific DNA methyltransferase by mediating methylation of DNA on the 6th position of adenine (N(6-methyladenosine. The existence of N(6-methyladenosine (m6A on DNA is however unclear in mammals, and additional evidences are required to confirm the role of the N(6-adenine-specific DNA methyltransferase activity of METTL4 in vivo. Acts as a regulator of mitochondrial transcript levels and mitochondrial DNA (mtDNA copy number by mediating mtDNA N(6-methylation: m6A on mtDNA reduces transcription by repressing TFAM DNA-binding and bending. N(6-methyladenosine deposition by METTL4 regulates Polycomb silencing by triggering ubiquitination and degradation of sensor proteins ASXL1 and MPND, leading to inactivation of the PR-DUB complex and subsequent preservation of Polycomb silencing (By similarity.,METTL4,HsT661,Epigenetics & Nuclear Signaling,METTL4

Molecular Weight: 54 kDa

Gene ID: 64863

UniProt: Q8N3J2