CD34 antibody

Catalog No. ABIN614812

This Mouse Monoclonal antibody specifically detects CD34 in FACS, WB, IP, IHC (fro) and Func. It exhibits reactivity toward Human and Primate.

Quick Overview for CD34 antibody (ABIN614812)

Target: CD34

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: QBEnd-10

Product Details anti-CD34 Antibody

Specificity: This antibody reacts with Class II epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker.

Cross-Reactivity (Details): Species reactivity (tested):Human, Non-Human Primates, negative on Rat, Bovine, Sheep, Canine (Dog)

Purification: Protein-A affinity chromatography

Purity: > 95 % pure by SDS-PAGE

Immunogen: Human endothelial vesicles

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 μm filter sterilized

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background: CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker

Gene ID: 947

NCBI Accession: NP_001020280

UniProt: P28906