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MCAM antibody (CF®405S)

This Mouse Monoclonal antibody specifically detects MCAM in IHC, FACS and IF. It exhibits reactivity toward Human.
Catalog No. ABIN6151287

Quick Overview for MCAM antibody (CF®405S) (ABIN6151287)

Target

See all MCAM Antibodies
MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity

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Human

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This MCAM antibody is conjugated to CF®405S

Application

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Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone

C146-634
  • Purpose

    Mouse Monoclonal anti-CD146 / MCAM / MUC18 / Mucin 18 (C146/634), CF405S Conjugate

    Characteristics

    The human CD146 gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18, that belongs to the immunoglobulin superfamily and functions as a Ca2 -independent cell adhesion molecule. CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. CD146 is found on 80 % of advanced primary human melanomas and correlates well with development of metastatic disease. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    Immunogen

    Recombinant human CD146 protein

    Isotype

    IgG1 kappa
  • Application Notes

    Immunohistology (formalin)
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 0.5-1.0 μg/mL
    • Optimal dilution for a specific application should be determined by user

    Comment

    A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    100 μg/mL

    Buffer

    PBS/0.1 % BSA/0.05 % azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Protect from light
  • Target

    MCAM (Melanoma Cell Adhesion Molecule (MCAM))

    Alternative Name

    CD146 / MCAM / MUC18 / Mucin 18

    Molecular Weight

    130 kDa

    Gene ID

    4162, 599039

    UniProt

    P43121
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