MCAM antibody (CF®405S)
Quick Overview for MCAM antibody (CF®405S) (ABIN6151287)
Target
See all MCAM AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- Mouse Monoclonal anti-CD146 / MCAM / MUC18 / Mucin 18 (C146/634), CF405S Conjugate
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Characteristics
- The human CD146 gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18, that belongs to the immunoglobulin superfamily and functions as a Ca2 -independent cell adhesion molecule. CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. CD146 is found on 80 % of advanced primary human melanomas and correlates well with development of metastatic disease. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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Immunogen
- Recombinant human CD146 protein
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Isotype
- IgG1 kappa
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Application Notes
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Immunohistology (formalin)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 0.5-1.0 μg/mL
- Optimal dilution for a specific application should be determined by user
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Comment
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A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 100 μg/mL
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Buffer
- PBS/0.1 % BSA/0.05 % azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Protect from light
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- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
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Alternative Name
- CD146 / MCAM / MUC18 / Mucin 18
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Molecular Weight
- 130 kDa
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Gene ID
- 4162, 599039
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UniProt
- P43121
Target
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