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CD44 Standard antibody (CF®647)

CD44s Reactivity: Human, Non-Human Primate FACS, IF, IHC (f) Host: Mouse Monoclonal HCAM-918 CF®647
Catalog No. ABIN6151561
  • Target See all CD44 Standard (CD44s) Antibodies
    CD44 Standard (CD44s)
    Reactivity
    • 30
    • 12
    • 9
    • 9
    • 8
    • 6
    Human, Non-Human Primate
    Host
    • 35
    • 1
    Mouse
    Clonality
    • 36
    Monoclonal
    Conjugate
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD44 Standard antibody is conjugated to CF®647
    Application
    • 36
    • 30
    • 29
    • 10
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Purpose
    Mouse Monoclonal anti-CD44 Standard (HCAM/918), CF647 Conjugate
    Characteristics
    This antibody recognizes a cell surface glycoprotein of 80-95 kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95 kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Recombinant human HCAM protein
    Clone
    HCAM-918
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product CD44s Primary Antibody
  • Application Notes
    Immunohistology formalin-fixed 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    DU145 cells. Tonsil, lymph node or breast carcinomas.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    CD44 Standard (CD44s)
    Alternative Name
    CD44 Standard (CD44s Products)
    Molecular Weight
    80-95 kDa
    Gene ID
    960, 502328
    UniProt
    P16070
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