CD59 antibody (CF®594)

Catalog No. ABIN6151691

This anti-CD59 antibody is a Mouse Monoclonal antibody detecting CD59 in FACS and IF. Suitable for Human.

Quick Overview for CD59 antibody (CF®594) (ABIN6151691)

Target: CD59

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD59 antibody is conjugated to CF®594

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: BRA-10G

Product Details anti-CD59 Antibody (CF®594)

Purpose: Mouse Monoclonal anti-CD59 / Protectin Heavy Chain (BRA-10G), CF594 Conjugate

Characteristics: This antibody reacts with human CD59, a 20 kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb is useful for study on GPI-anchored proteins, PNH and CD59 functions. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human K562 tumor cells

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Daudi, CEM, K562, HPB.ALL, Jurkat, Raji, and human lymphocytes. Human lymph node and tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD59

Target: CD59

Alternative Name: CD59 (heavy chain of Protectin)

Molecular Weight: 20 kDa

Gene ID: 966, 278573

UniProt: P13987

Pathways: Complement System