Integrin beta 3 antibody (CF®488A)

Catalog No. ABIN6151803

This Mouse Monoclonal antibody specifically detects Integrin beta 3 in FACS, IHC (fro) and IF. It exhibits reactivity toward Human.

Quick Overview for Integrin beta 3 antibody (CF®488A) (ABIN6151803)

Target: Integrin beta 3 (ITGB3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 3 antibody is conjugated to CF®488A

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Clone: ITGB3-1713

Product Details anti-Integrin beta 3 Antibody (CF®488A)

Purpose: Mouse Monoclonal anti-CD61 / Integrin-beta-3 / Platelet Glycoprotein IIIa (ITGB3/1713), CF488A Conjugate

Characteristics: This antibody reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain). It associates with the ?V-chain (CD51) to form vitronectin receptor, or with the ?IIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61). The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human ITGB3 protein

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry 0.5-1 μg/million cells in 0.1 mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Immunohistology (frozen or formalin) 1-2 μg/mL
• Immunofluorescence 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

U937, KG1a, HEL cells. Spleen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: CD61 / Integrin-beta-3 / Platelet Glycoprotein IIIa

Background: BDPLT2, GP3A, GPIIIa, GT, HPA 1, HPA 4, Integrin beta-3, ITGB3, NAIT, Platelet fibrinogen receptor beta subunit, Platelet glycoprotein IIIa, PTP

Molecular Weight: 105 kDa & 90 kDa

Gene ID: 3690, 218040

UniProt: P05106

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex