GLG1 antibody (CF®647)

Catalog No. ABIN6152625

This Mouse Monoclonal antibody specifically detects GLG1 in WB, ICC, FACS, IF and IHC (f). It exhibits reactivity toward Human.

Quick Overview for GLG1 antibody (CF®647) (ABIN6152625)

Target: GLG1 (Golgi Glycoprotein 1 (GLG1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GLG1 antibody is conjugated to CF®647

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: GLG1-970

Product Details anti-GLG1 Antibody (CF®647)

Purpose: Mouse Monoclonal anti-GLG1 (Golgi Complex) (GLG1/970), CF647 Conjugate

Characteristics: This MAb recognizes a protein of 134 kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Golgi fraction from human liver cells

Isotype: IgG1 kappa

Application Details

Application Notes: Does not react with mouse or rat, others not known

• Immunohistology formalin-fixed 0.5-1 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunocytochemistry Acetone or paraformaldehyde fixed 0.5-1 μg/mL
• Immunofluorescence 0.5-1 μg/mL
• Western blotting 0.5-1.0 μg/mL
• Flow Cytometry 0.5-1.0 μg/million cells in 0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

HepG2, A431 or HeLa cells. Placenta, Tonsil, Testis and Ovary.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for GLG1

Target: GLG1 (Golgi Glycoprotein 1 (GLG1))

Alternative Name: GLG1 (Golgi Complex)

Molecular Weight: 134 kDa

Gene ID: 2734, 109731

UniProt: Q92896