LGALS1/Galectin 1 antibody (AA 12-108) (CF®640R)

Catalog No. ABIN6152659

The Mouse Monoclonal anti-LGALS1/Galectin 1 antibody has been validated for WB, IHC, ELISA, FACS, IF and Coat. It is suitable to detect LGALS1/Galectin 1 in samples from Human.

Quick Overview for LGALS1/Galectin 1 antibody (AA 12-108) (CF®640R) (ABIN6152659)

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LGALS1/Galectin 1 antibody is conjugated to CF®640R

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat)

Clone: GAL1-1831

Product Details anti-LGALS1/Galectin 1 Antibody (CF®640R)

Binding Specificity: AA 12-108

Purpose: Mouse Monoclonal anti-Galectin-1 / Human Placental Lactogen (GAL1/1831), CF640R Conjugate

Characteristics: Galectin-1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14 kD participating in a variety of normal and pathological processes, including cancer progression. Galectin-1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin-1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 Molecules present on the surface of lymphocytes. Galectin-1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Galectin-1 protein fragment (aa12-108) (exact sequence is proprietary)

Isotype: IgG Mix lambda

Application Details

Application Notes: Flow cytometry 0.5-1 μg/million cells in 0.1 mL

• Immunohistology (formalin) 0.5-1 μg/mL
• ELISA 2-4 μg/mL for coating (order Ab without BSA)
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa, K562 or 293 cells. Prostate, Kidney, Placenta, Stomach, Skin, Spleen, Brain or Heart.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for LGALS1/Galectin 1

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Alternative Name: Galectin-1 / Placental Lactogen

Molecular Weight: 14 kDa

Gene ID: 3956, 445351

UniProt: P09382

Pathways: Carbohydrate Homeostasis