VWF antibody (AA 1815-1939) (CF®640R)

Catalog No. ABIN6154597

The Mouse Monoclonal anti-VWF antibody has been validated for WB, IHC, IP, FACS and IF. It is suitable to detect VWF in samples from Human.

Quick Overview for VWF antibody (AA 1815-1939) (CF®640R) (ABIN6154597)

Target: VWF (Von Willebrand Factor (VWF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VWF antibody is conjugated to CF®640R

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: VWF-1465

Product Details anti-VWF Antibody (CF®640R)

Binding Specificity: AA 1815-1939

Purpose: Mouse Monoclonal anti-von-Willebrand Factor / Factor-VIII-Related Ag (VWF/1465), CF640R Conjugate

Characteristics: von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human vWF protein (aa1815-1939) (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• Western blotting 0.5-1 μg/mL Immunoprecipitation 0.5-1 μg/500 μg protein lysate
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

HUVEC cells or Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for VWF

Target: VWF (Von Willebrand Factor (VWF))

Alternative Name: von-Willebrand Factor / Factor-VIII-Related Ag

Background: Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebr, Antigen 2, von Willebr, Disease (vWD)

Molecular Weight: 250 kDa

Gene ID: 7450, 440848

UniProt: P04275