AMPD3 antibody (CF®594)

Catalog No. ABIN6155529

This Mouse Monoclonal antibody specifically detects AMPD3 in IF, ICC, FACS and IHC (f). It exhibits reactivity toward Human.

Quick Overview for AMPD3 antibody (CF®594) (ABIN6155529)

Target: AMPD3 (Adenosine Monophosphate Deaminase 3 (AMPD3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AMPD3 antibody is conjugated to CF®594

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: AMPD3-901

Product Details anti-AMPD3 Antibody (CF®594)

Purpose: Mouse Monoclonal anti-Adenosine Monophosphate Deaminase-3 (AMPD3) (AMPD3/901), CF594 Conjugate

Characteristics: This antibody recognizes a protein of ~90 kDa, which is identified as Adenosine Monophosphate Deaminase, isoform E (AMPD3). It has 767 amino acids and is assigned an EC 3.5.4.6. It is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. AMPD3 gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. This MAb shows reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. This MAb is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human AMDP3 protein

Isotype: IgG2b kappa

Application Details

Application Notes: Immunohistology formalin-fixed 2-4 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunocytochemistry 1-2.0 μg/mL
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 1-2 μg/million cells in 0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

RBC. Fetal liver, Spleen or Placenta

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for AMPD3

Target: AMPD3 (Adenosine Monophosphate Deaminase 3 (AMPD3))

Alternative Name: Adenosine Monophosphate Deaminase-3 (AMPD3)

Molecular Weight: 90 kDa

Gene ID: 272, 501890

UniProt: Q01432