Aurora Kinase B antibody (AA 89-251) (CF®568)

Catalog No. ABIN6155557

The Mouse Monoclonal anti-Aurora Kinase B antibody has been validated for WB, IF and FACS. It is suitable to detect Aurora Kinase B in samples from Human.

Quick Overview for Aurora Kinase B antibody (AA 89-251) (CF®568) (ABIN6155557)

Target: Aurora Kinase B (AURKB)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Aurora Kinase B antibody is conjugated to CF®568

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: AURKB-1521

Product Details anti-Aurora Kinase B Antibody (CF®568)

Binding Specificity: AA 89-251

Purpose: Mouse Monoclonal anti-Aurora-B (AURKB/1521), CF568 Conjugate

Characteristics: This antibody recognizes a protein of 39 kDa, which is identified as Aurora B. The serine/threonine protein kinase aurora B (Aurora B) is a chromosomal passenger protein critical for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Aurora B protein fragment (aa89-251) (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa cells. Tonsil, Placenta, Hodgkin s Lymphoma or Colon Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: Aurora-B

Molecular Weight: 39 kDa

Gene ID: 9212, 442658

UniProt: Q96GD4

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades